Pc-12 Cells, Pc12 Cell Line Wikipedia
We investigated the mechanisms involved in intracellular Ca2 signaling in PC-12 cells. A CELL LINE derived from a PHEOCHROMOCYTOMA of the rat ADRENAL MEDULLA.
Identification Of Biochemical And Cytotoxic Markers In Cocaine Treated Pc12 Cells Scientific Reports
F-12K 25 FBS 15 Horse Serum.
Pc-12 cells. Break up cell clusters by gently aspirating cells through a 22g needle 4 or 5 times. ATCC has not tested CRL-17211 cells for differentiation potential in response to NGF treatment. Remove approximately twothirds of the culture1 medium from each plate and replace with fresh complete growth medium 10 ml for 150-mm dishes.
Every step of the way a wide range of cell health products Maintaining healthy cells is the key to experimental success and reproducible research results. When cells are grown with collagen - neuronal fibroblasts. 1163 The cells respond reversibly to NGF by induction of the neuronal phenotype.
Used in neurobiological and neurochemical studies. PC12 cells resemble the phenotype of sympathetic ganglion neurons upon differentiation with nerve growth factor NGF and can be subcultured indefinitely. Cells synthesise and store catecholamine dopamine and NPP.
Rattus norvegicus rat Tissue. The effect of LPS treatment on PC-12 cells. A PC-12 cell viability after LPS treatment was detected by CCK-8.
The delayed Ca2 release was dependent on prior ATP priming and on dopamine secretion by PC-12 cells. Used in neurobiological and neurochemical studies. PC12 PC 12 Rat adrenal phaeochromocytoma Derived from a transplantable rat adrenal phaeochromocytoma.
Remove cells from N2 and rapidly thaw in a 37oC waterbath with constant agitation Should take about 2. Be the first to write a review. Due to their differentiation ability and ease of culture PC-12 cells are used in a variety of research areas ranging from drug efficacy to neurosecretion.
The PC-12 cell line was derived from a transplantable rat pheochromocytoma. PC-12 cell line from rat has been used to create artificial nervous system tissue models to study neurodegeneration. The medium should be added gently and from the side of the tissue culture dish.
1163 The cells do not synthesize epinephrine. Derived from a transplantable rat adrenal phaeochromocytoma. PC12 cells appear to possess specific receptors for and responses to epidermal growth factor EGF Huff and Guroff 1979.
C D Expression levels of inflammatory cytokines after LPS treatment were detected by ELISA. This adherent variant PC-12 Adh has been adapted to Corning CellBIND flasks to improve cell attachment. PC-12 cells were treated with H 2 O 2 to cell injury.
PC12 cells a rat pheochromocytoma cell line and Neuro2A cells a mouse neuroblastoma cell line are commonly used model systems to study the pathways involved in early neuronal differentiation. Please refer to the ATCC CRL-1721 cell line which responds reversibly to NGF by induction of the neuronal phenotype. Feed PC 12 cells three times a week with complete growth medium.
B PC-12 cell apoptosis after LPS treatment was detected by flow cytometry. Pheochromocytoma PC 12 is another type of cell that has been used for amperometric studies. A PC-12 cell viability after LPS treatment was detected by CCK-8.
PC12 cells stop dividing and undergo terminal differentiation when treated with NERVE GROWTH FACTOR making the line a. Remove and discard supernatant. Cell Culture Growth ConditionsProtein ExpressionRNAiStable Cell TransfectionTransient TransfectionVirus Production.
To give you confidence in the health of your cells every step of the way weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell. Find the latest published documents for Pc 12 Cells Related hot topics top authors the most cited documents and related journals. View StainFree Cell Detection Webinar.
Resuspend cells in 5 mL complete growth medium. Under different transfected treatments cell viability migration invasion and. C D Expression levels of inflammatory cytokines after LPS treatment were detected by ELISA.
PC-12 Cell Line Protocol General Information. It has also been used to perform cell contact assay to test the biocompatibility of high-density electrode arrays. 15 ml for 35- mm dishes.
Transfer the vial contents to a 50 ml tube containing 9 mL complete growth medium. The PC-12 cell line was derived from a transplantable rat pheochromocytoma. Cells were purchased from ATCC Catalog CRL-1721 Cells are stored in liquid N2 tank Rack 1 Box A Culturing Maintenance.
B PC-12 cell apoptosis after LPS treatment was detected by flow cytometry. Adherent small clusters Strain. Centrifuge cells at 180 - 225 x g for 8-15 minutes at room temperature.
The cells were transfected with short-hairpin RNA directed against Sox2ot sh-Sox2ot small interfering RNA directed against myeloid cell leukemia-1 MCL-1 isoform2 si-MCL-1 a miR-211 mimic a miR-211 inhibitor and their negative controls. Cells synthesise and store catecholamine dopamine and NPP. As an established cell line PC12 cells share the general advantages conferred by the capacity for unlimited proliferation.
Neurobiological and neurochemical studies. The presence of such receptors could reflect a hitherto unidentified role of EGF during neuronal development or could alternatively correlate with the neoplastic nature of PC12 cells. Like adrenal chromaffin cells PC12 cells synthesize and store DA and sometimes noradrenaline which are released upon depolarization in a Ca 2-dependent way Greene Rein 1977.
In response to nerve growth factor NGF a neuronal phenotype can be reversibly induced. The stimulation of NGF-differentiated PC-12 cells with 3μM ATP caused an early Ca2 release followed by a delayed Ca2 release. Respond reversibly to nerve growth factor by induction of neuronal phenotype.
5 ml for 100-mm dishes. Respond reversibly to nerve growth factor by induction of neuronal phenotype. One aspect of these cells that is of particular interest is that.
The effect of LPS treatment on PC-12 cells. They can be produced in large numbers with a minimum of effort and since they are derived from a single progenitor represent a relatively homogeneous population that can be used to provide replicate cultures. PC-12 cells are able to differentiate into neuron-like cells in the presence of nerve growth factor or dexamethasone.
Pc 12 Adh Atcc
Pc12 Cell Line Wikipedia
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